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Please use this identifier to cite or link to this item: http://hdl.handle.net/2031/4309

Title: Antioxidant responses and lipid peroxidation in gold-lined sea bream (rhabdosargus sarba) upon exposure to chattonella marina, a red tide causative species
Other Titles: Chi chao hai yang he bao zao dui jin si yu kang yang hua fan ying ji zhi zhi guo yang hua zhi ying xiang
赤潮海洋褐胞藻對金絲魚抗氧化反應及脂質過氧化之影響
Authors: Woo, Pui Shan (胡珮珊)
Department: Dept. of Biology and Chemistry
Degree: Master of Philosophy
Issue Date: 2004
Publisher: City University of Hong Kong
Subjects: Fishes -- Effect of water pollution on
Red tide
Water -- Pollution -- Toxicology
Notes: CityU Call Number: SH174.W66 2004
Includes bibliographical references (leaves 115-140)
Thesis (M.Phil.)--City University of Hong Kong, 2004
xx, 140 leaves : ill. (some col.) ; 30 cm.
Type: Thesis
Abstract: There has been a global increase in the frequency and spatial scale of red tides. Chattonella spp. which has caused mass mortality of cultured fish in many parts of the world frequently present in coastal waters. C. marina is known to produce high concentrations of reactive oxygen species (ROS), including O2.-, H2O2 and OH.. Several workers therefore, postulated that oxidative stress caused by ROS produced is the main cause of fish-kill by this species. Since ROS is being produced endogenously during aerobic metabolism, a variety of antioxidant enzymes (e.g. superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GR)) and free radical scavengers (glutathione (GSH)) have been evolved in aerobic organisms, to remove ROS. However, when these antioxidant responses are overwhelmed by ROS, oxidative damages such as lipid peroxidation (LPO), protein oxidation and DNA-oxidation may occur. We hypothesize that these antioxidant responses will occur when fish is being challenged with oxidative stress caused by C. marina. Using the gold-lined sea bream (Rhabdosargus sarba) as a model, this thesis aims to study: (1) to investigate the responses of antioxidant enzymes and induction of lipid peroxidation in gill and erythrocytes of fish upon exposure to sub-bloom and bloom levels of C. marina, (2) to test the hypothesis that ROS is the main toxic mechanism of C. marina that lead to fish kill, and (3) to explore the possibility of using some of the antioxidant responses as biomarker(s) for oxidative stress induced by C. marina. C. marina produced high levels of H2O2 (9.69 ± 0.19 μM in 2,000 cells/ml and 18.95 ± 1.01 μM in 8,000 cells/ml), which were significantly higher than those in algal (D. tertiolecta) control (~ 1 μM) and seawater control (~ 1 μM). Despite this, no significant changes in antioxidant enzymes, antioxidant scavengers and lipid peroxidation were found in gill and erythrocytes after fish was exposed to high concentration (for 1 & 2 h) and low concentration (for 3 & 6 h) of C. marina. In second part of this study, experiments were also carried out, in which fish were exposed to some 0.5 mM and 1 mM of H2O2 for 1 to 5 h. (equivalent to 25 to 50 times higher than that (~ 20 μM) produced by C. marina). No alterations in SOD was observed in gill, while significant changes in CAT, GPx, GR activities and [GSH]/[GSSG] were observed in the gill upon exposure to 1 mM H2O2 (for 1 & 2 h) and 0.5 mM (for 3 & 5 h). In contrast, no change in GPx, GR activities and [GSH]/[GSSG] were observable in erythrocytes, while SOD and CAT activities in erythrocytes decreased when exposed to both concentrations of H2O2. Lipid peroxidation was not found in both gill and erythrocytes after exposed to 1 mM (for 1 & 2 h) H2O2 and 0.5 mM (for 3 & 5 h). The fact that neither antioxidant response nor lipid peroxidation was found when fish were exposed to C. marina and much higher concentrations of H2O2 provide unequivocal evidence to show that ROS produced by C. marina is not the main cause of ichthyotoxicity in fish kills. A critical analysis of literature data also showed no convincing evidence to suggest that other toxic mechanisms such as impairment of oxygen transfer due to physical gill damage, toxicity caused by production of brevetoxin-like compounds and polyunsaturated fatty acid (PUFA) are involved on fish kill caused by C. marina.
Online Catalog Link: http://lib.cityu.edu.hk/record=b1871334
Appears in Collections:BCH - Master of Philosophy

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